It looks like the file is locked by some other process. This could happen if when reading/writing to it you forgot to dispose the stream reader/writer and you leaked the unmanaged handler to the file.
For example if you used the following code to read from the file:
StreamReader reader = new StreamReader(fileName);
string contents = reader.ReadToEnd();
and you never release the reader, the file will be locked. The proper way is to wrap IDisposable resources such as Streams and StreamReaders in using statements:
using (StreamReader reader = new StreamReader(fileName))
{
string contents = reader.ReadToEnd();
}
If on the other hand the file is locked by some other external process to your application then there's very little you could do about it, other than killing this process.