I am plotting scatterplot using ggplot for 10 subjects and each subject contain 2 values in two different columns. I am able to plot scatter plot but not been able to adjust legend for it. Legend box should show donor-1, donor-2..., donor-10, but instead it is showing donor-1, donor-10, ...., donor-9. Also, it is showing block of size mentioned in geom_point function in legend which should not be there.
Below is my code snippet for it:
color1 = 'red'
color2 = 'green'
color3 = 'blue'
color4 = 'forestgreen'
color5 = 'purple'
color6 = 'yellow'
color7 = 'orange'
color8 = 'royalblue'
color9 = 'palevioletred4'
color10 = 'pink'
p2<-qplot(CEACAM4.2hrs, CEACAM4.EDTA, data=res, xlab="CECAM4 PAX 2 Hr", ylab=" CECAM4 EDTA 0 Hr")
p3<-p2+geom_point(aes(color = row.names(res2), size = 3)) + scale_x_continuous(limit =c(22,28), breaks = c(22,23,24,25,26,27,28)) + scale_y_continuous(limit=c(22,28), breaks = c(22,23,24,25,26,27,28)) + stat_smooth(method="lm", se=FALSE)+ scale_color_manual(values=c(color1, color2, color3, color4, color5, color6, color7, color8, color9, color10))
p4<- p3 + guides(colour = guide_legend(override.aes = list(size = 10)))
p4+ ggtitle("CECAM4 EDTA 0Hr Vs PAX 2Hr") + theme(plot.title = element_text(size = 28,colour=" mediumvioletred", face = "bold"))+ theme(legend.key = element_rect(colour = "black"), legend.title=element_text(size=22), legend.text=element_text(size=20)) + theme(plot.margin=unit(c(2.5,2,2,2),"cm"))+ theme(axis.text=element_text(size=18, face="bold"), axis.title=element_text(size=20, face="bold", colour="purple4")) + theme(axis.title.y=element_text(vjust=-0.65)) + theme(axis.title.x=element_text(vjust=-3.0)) + theme(plot.title = element_text(vjust=5)) + theme(axis.ticks.length=unit(1,"cm")) + theme(legend.key.size=unit(1.5,"cm"))
ggsave(file="ggplot_cecam4_2_edta.png")
So, question more precisely is, how can I get legend in sequence with ggplot? e.g.
Donor-1
Donor-2
Donor-3
Donor-4
Donor-5
Donor-6
Donor-7
Donor-8
Donor-9
Donor-10
Thank you..