Neither the Java Language Specification nor the Java Virtual Machine Specification specifies the answer to this because it's an implementation detail. In fact, JVMS §2.7 specifically says:
Representation of Objects
The Java Virtual Machine does not mandate any particular internal structure for objects.
In theory, a conformant virtual machine could implement objects which have a lot of fields using set of bit flags to mark which fields have been set to non-default values. Initially no fields would be allocated, the flag bits would be all 0, and the object would be small. When a field is first set, the corresponding flag bit would be set to 1 and the object would be resized to make space for it. [The garbage collector already provides the necessary machinery for momentarily pausing running code in order to relocate live objects around the heap, which would be necessary for resizing them.]
In practice, this is not a good idea because even if it saves memory it is complicated and slow. Access to fields would require temporarily locking the object to prevent corruption due to multithreading; then reading the current flag bits; and if the field exists then counting the set bits to calculate the current offset of the wanted field relative to the base of the object; then reading the field; and finally unlocking the object.
So, no general-purpose Java virtual machine does anything like this. Some objects with an exorbitant number of fields might benefit from it, but even they couldn't rely on it, because they might need to run on the common virtual machines which don't do that.
A flat layout which allocates space for all fields when an object is first instantiated is simple and fast, so that is the standard. Programmers assume that objects are allocated that way and thus design their programs accordingly to best take advantage of it. Likewise, virtual machine designers optimize to make that usage fast.
Ultimately the flat layout of fields is a convention, not a rule, although you can rely on it anyway.