When you are in Multiline TextMode, MaxLength has no effect on that TextBox control. So instead of that your are suppose to use a client side validation that will check your input string length on every keypress and restrict you from typing extra characters when your input exceeds maximum length using event.preventDefault() that restricts the default behavior of that keypress event. (You can also go with regular expression as mentioned in other answer)
Add this script in the head section :
<script src="http://code.jquery.com/jquery-2.1.1.min.js"></script>
<script>
$(document).ready(function () {
$("#txtComment").on("keypress", function (e) {
if ($(this).val().length == 250) {
e.preventDefault();
}
});
});
</script>
Note : If you want to add this validation script to multiple controls then mark all the controls with same class name and in your script replace the id of the control with that class name for all controls.
References : .keypress() , event.preventDefault()