Getting the delta between two datetime objects in Python is really simple, you simply subtract them.
import datetime
d1 = datetime.datetime.now()
d2 = datetime.datetime.now()
delta = d2 - d1
print delta.total_seconds()
d2 - d1
returns a datetime.timedelta object, from which you can get the total second difference between the two dates.
As for formatting the dates, you can read about formatting strings into datetime objects, and datetime objects into string here
You'll read about the strftime() and strptime() functions, and with them you can get yourself two datetime objects which you can subtract from each other.