The elasticbeanstalk-access_log log-file in our AWS EBS instances are full of 408 errors, like these:
172.31.1.56 (-) - - [16/Mar/2016:10:16:31 +0000] "-" 408 - "-" "-"
172.31.1.56 (-) - - [16/Mar/2016:10:16:31 +0000] "-" 408 - "-" "-"
172.31.1.56 (-) - - [16/Mar/2016:10:16:31 +0000] "-" 408 - "-" "-"
172.31.1.56 (-) - - [16/Mar/2016:10:16:31 +0000] "-" 408 - "-" "-"
172.31.1.56 (-) - - [16/Mar/2016:10:16:31 +0000] "-" 408 - "-" "-"
172.31.1.56 (-) - - [16/Mar/2016:10:16:59 +0000] "-" 408 - "-" "-"
They appear randomly, sometimes there are a few minutes between them, sometimes there are 4-6 errors within a few seconds. These errors also happen on our non-public staging environment when there is no any real traffic on the server, so the source of these requests is probably one of AWS's own service.