I believe what you are after is called a Projective Transformation. The link below should guide you through exactly what you need.
Demonstration of calculating a projective transformation with proper math typesetting on the Math SE.
Although you can solve this by hand and write that into your code... I strongly recommend using a matrix math library or even writing your own matrix math functions prior to resorting to hand calculating the equations as you will have to solve them symbolically to turn it into code and that will be very expansive and prone to miscalculation.
Here are just a few tips that may help you with clarification (applying it to your problem):
-Your A matrix (source) is built from the 4 xy points in your camera image (pixel locations).
-Your B matrix (destination) is built from your measurements in in the real world.
-For fast recalibration, I suggest marking points on the ground to be able to quickly place the cube at the 4 locations (and subsequently get the altered pixel locations in the camera) without having to remeasure.
-You will only have to do steps 1-5 (once) during calibration, after that whenever you want to know the position of something just get the coordinates in your image and run them through step 6 and step 7.
-You will want your calibration points to be as far away from eachother as possible (within reason, as at extreme distances in a vanishing point situation, you start rapidly losing pixel density and therefore source image accuracy). Make sure that no 3 points are colinear (simply put, make your 4 points approximately square at almost the full span of your camera fov in the real world)
ps I apologize for not writing this out here, but they have fancy math editing and it looks way cleaner!
Final steps to applying this method to this situation:
In order to perform this calibration, you will have to set a global home position (likely easiest to do this arbitrarily on the floor and measure your camera position relative to that point). From this position, you will need to measure your object's distance from this position in both x and y coordinates on the floor. Although a more tightly packed calibration set will give you more error, the easiest solution for this may simply be to have a dimension-ed sheet(I am thinking piece of printer paper or a large board or something). The reason that this will be easier is that it will have built in axes (ie the two sides will be orthogonal and you will just use the four corners of the object and used canned distances in your calibration). EX: for a piece of paper your points would be (0,0), (0,8.5), (11,8.5), (11,0)
So using those points and the pixels you get will create your transform matrix, but that still just gives you a global x,y position on axes that may be hard to measure on (they may be skew depending on how you measured/ calibrated). So you will need to calculate your camera offset:
object in real world coords (from steps above): x1, y1
camera coords (Xc, Yc)
dist = sqrt( pow(x1-Xc,2) + pow(y1-Yc,2) )
If it is too cumbersome to try to measure the position of the camera from global origin by hand, you can instead measure the distance to 2 different points and feed those values into the above equation to calculate your camera offset, which you will then store and use anytime you want to get final distance.