My current system for CUDA applications has one old NVIDIA card, 8800 GTX. I am thinking of adding one more card to it without updating the motherboard. Is it true that as long as I have two PCI-E slots, the two will work? Or I have to purchase a new motherboard enabled SLI support?
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Of course you can. Moreover, even if one use multiple GPUs on SLI configuration - for CUDA they will be shown as multiple devices. For example, I have a computer with 4 nVidia GPUs on AMD-chip motherboard w/o any SLI support.

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