Just because a jdbc connection from Data-Studio is working, it does not mean that your pyodbc will connect to Db2. pyodbc does not use jdbc, instead it will use the CLI/ODBC interface to Db2 which gets implemented by a suitable driver.
To use Db2 from pyodbc on Windows, ensure you first have either a Db2-client installed on Windows, or a Db2-server installed installed on Windows.
There are many kinds of Db2-clients. Much depends on the operating-system that runs your Db2-server (Z/OS, i-Series, Linux, Unix, Windows), and what kind of activities you want to perform on the client (developing, administering/monitoring, querying, or all three).
For some target platforms there are non-IBM drivers, but I won't discuss those.
In python, you can choose to connect to a database either with a DSN (Data Source Name) (usually this involves a shorter connection string)
or without a DSN (longer connection string containing all the details).
When learning, it may be easier to get Microsoft Windows to do most of the initial work with odbcad32.
This is most easy if the Db2-client is already configured to access one or more Db2-databases . The prereq is that the driver supports CLI/ODBC.
To define a DSN (either a system-DSN or a user-DSN) use the Microsoft odbcad32 tool to point to your Db2 database and verify connectivity.
If the Db2-server runs on Z/OS or i-Series, special licensing requirements may apply depending on whether you are directly connecting to the target Db2-server or whether you are using a Db2-connect gateway.
Take a note of the exact DRIVER string inside odbcad32 for the Db2-database, including case and spaces because you will need that in your python code.
For example, that driver name might look like 'IBM DB2ODBC DRIVER - DB2COPY1' if you have a local Db2-server installed on Windows (such as the free Db2-Express-C).
Verify that the connection to the Db2-database is successful inside odbcad32. That is crucial.
When odbcad32 succeeds to connect then pyodbc will succeed to connect usually.
In your python code, your connection-string can either use the DSN or explicitly quote the DRIVER/SERVER/PORT/DATABASE/UID/PWD, along with any other required settings on the connection string.
Remember also that you don't have to use pyodbc. There are other options for python to interact with Db2-Servers. Make an informed choice and do your research.
You can also use the IBM supplied module "ibm_db" or the DBI interface module "ibm_db_api", or if you are using an object relational mapper you can use the SQLAlchemy adapter (ibm_db_sa), or you can use django framework.
Read all about that in the Db2 documentation here.
https://www.ibm.com/support/knowledgecenter/en/SSEPGG_10.5.0/com.ibm.swg.im.dbclient.python.doc/doc/c0054366.html