EDIT: Since hek2mgl sir mentioned in case you need to remove continuous similar lines then try following.
Let's say following is Input_file:
cat Input_file
chr1 109472560 109472561 -4732 CLCC1
chr1 109472560 109472561 -4732 CLCC1
chr1 109477498 109477499 206 CLCC1
chr1 109477498 109477499 206 CLCC1
chr1 109477498 109477499 206 CLCC1
chr1 109472560 109472561 -4732 CLCC1
chr1 109477498 109477499 206 CLCC1
chr1 109472560 109472561 -4732 CLCC1
Run following code now:
awk 'prev!=$0;{prev=$0}' Input_file
Output will be as follows.
chr1 109472560 109472561 -4732 CLCC1
chr1 109477498 109477499 206 CLCC1
chr1 109472560 109472561 -4732 CLCC1
chr1 109477498 109477499 206 CLCC1
chr1 109472560 109472561 -4732 CLCC1
The following snippet will remove all duplicate lines, not only repeating lines
awk '!a[$0]++' Input_file
Append > output_file
to above command in case you want to take output into a separate file.
Explanation: Adding explanation for above code now. This is only for explanation purposes for running code use above mentioned one only.
awk '
!a[$0]++ ##Checking condition here if current line is present in array a index or NOT, if not then increase its value by 1.
##So that next time it will make condition as FALSE, since we need to have only unique lines.
##awk works on method of condition and action, so if condition is TRUE it will do some action mentioned by programmer.
##Here I am not mentioning action so by default print of current line will happen, whenever condition is TRUE.
' Input_file ##mentioning Input_file name here.