You can attach a function as member data to another function (which is what is done in your example).
const express = () => {};
express.static = argument => console.log('argument');
express.static('public'); # console >>> 'public'
However, you cannot readily access a variable that is defined in a function body.
const express = () => {
const static = argument => console.log('argument');
};
express.static('public'); # console >>> Error: undefined in not a function
There is a signifiant difference between member data attached to a function (the first example) and the closure that wraps the body of the function (the second example).
So, to answer your question "is it possible in JavaScript to call a function which is inside a function?" No, this is not readily possible with the important note that this is not what is being done in your example.