How do I rewrite the following to properly replace the variable with my genomeID? (I have it working with this method in Spades and Masurca assemblers, so it's something about Abyss that doesnt like this approach and I need a work-around)
I am trying to run abyss on a cluster server but am running into trouble with how abyss-pe is reading my variable input:
- my submit file loads a script for each genome listed in a .txt file
- my script writes in the genome name throughout the script
- the abyss assembly fumbles the variable replacement
Input.sub:
queue genomeID from genomelisttest.txt
Input.sh:
#!/bin/bash
genomeID=$1
cp /mnt/gluster/harrow2/trim_output/${genomeID}_trim.tar.gz ./
tar -xzf ${genomeID}_trim.tar.gz
rm ${genomeID}_trim.tar.gz
for k in `seq 86 10 126`; do
mkdir k$k
abyss-pe -C k$k name=${genomeID} k=$k lib='pe1 pe2' pe1='../${genomeID}_trim/${genomeID}_L1_1.fq.gz ../${genomeID}_trim/${genomeID}_L1_2.fq.gz' pe2='../${genomeID}_trim/${genomeID}_L2_1.fq.gz ../${genomeID}_trim/${genomeID}_L2_2.fq.gz'
done
Error that I get:
`../enome_trim/enome_L1_1.fq.gz': No such file or directory
This is where "enome" is supposed to replace with a five digit genomeID, which happens properly in the earlier part of the script up to this point, where abyss comes in.