1
An hard drive, from a logical point of view, can be seen as a sequence of "blocks" (the more common name is sectors). How these blocks are organized physically on the disks can be disregarded, but the driver must know someway how to get data of course, though you send to modern hd driver "high level" commands that, as far as you know, are not strongly related to where data physically are (you can say "read the block 123", but there's no extern evidence of where that block lives).
However this way you can "name" a block with a number, and say e.g. that block 0 is the MBR. Each block contains several bytes (512, 1024...). Not all used blocks contain actual data of a file, in fact there are metainformations of any sort, depending on the filesystem but even related to the "structure" of the hd (I mean, partitions).
A file located on an hd is not automatically loaded into memory, so it has no memory address. Once you read it, piece of it if not all are of course copied into the memory you give, which is not an intrinsic property of the file. (Filesystems retrieve the blocks belonging to the file and "show" them as we are used to see them, as a single "unit", the file)
Summarizing: files have no memory address. The physical address could be the set of blocks holding data (and metadata, like inodes ) of the file, or just the first block (but if a block of data is N, N+1 could not belong to the same file - the blocks need no to be one next to the other). To know them, you have to analyse the structure of the filesystem you use. I don't know if there's an API to retrieve them easily, but in the worst case you can analyse the source code of the filesystem... good luck!
2
C functions are translated into assembly. If you respect the C calling convention, you can write a "C function" directly in assembly. Try reading this and this for x86.
3
You can call windows API from asm. Forget MS-DOS, MS-DOS is dead, MS-DOS is not Windows, the cmd is a sort of "emulation"... indeed no, not an emulation but just a command line interface that resemble the one MS-DOS users was used to. But it is not exaclty the same, i.e. there are no MS-DOS system interrupt you can use. Iczelion's assembly tutorials, though old, could be an interesting resource. (If links expire, try with the wayback machine)
4
I do not own Win7 and never installed nasm on windows, so I can't say anything about.