Opening a file does not put a lock on it. In fact, if you needed to ensure that separate processes did not access a file simultaneously, all these processes would have to to cooperatively take special steps to ensure that only a single process accessed the file at one time (see Locking a file in Python). This can also be demonstrated by the following small program that purposely takes its time in reading a file to give another process (namely me with a text editor) a chance to append some data to the end of the file while the program is running. This program reads and outputs the file one byte at a time pausing .1 seconds between each read. During the running of the program I added some additional text to the end of the file and the program printed the additional text:
import time
with open('test.txt', "rb") as infile:
while True:
data = infile.read(1)
if data == b'':
break
time.sleep(.1)
print(data.decode('ascii'), end='', flush=True)
You can read your file in pieces and then join these pieces together if you need one single byte string. But this will not be as memory efficient as reading the file with a single read:
BLOCKSIZE = 64*1024 # or some other value depending on the file size
with open(source, "rb") as infile:
blocks = []
while True:
data = infile.read(BLOCKSIZE)
if data == b'':
break
blocks.append(data)
# if you need the data in one piece (otherwise the pieces are in blocks):
data = b''.join(blocks)