Using this code:
library(tidyverse)
df1 <- structure(list(Pool_1.sf = c(1.04654112319058, 0.908757359307814 ), Pool_10.sf = c(1.09143254057473, 0.763847944339038), Pool_11.sf = c(1.18750942376232, 0.970606492107592), Pool_12.sf = c(1.40039457695623, 0.987404435084985 ), Pool_13.sf = c(1.40110264045617, 1.05184583712403), Pool_14.sf = c(0.975272918230445, 1.05978729086064), Pool_15.sf = c(1.18870377730237, 1.350632186878 ), Pool_16.sf = c(1.25322756941453, 1.18822754009871), Pool_17.sf = c(1.29750766166164, 0.561735111884727), Pool_18.sf = c(1.27533917021409, 1.38423737777244 ), Pool_19.sf = c(0.918846998358604, 0.95201145069032), Pool_2.sf = c(0.748425883461423, 0.618517135274528), Pool_20.sf = c(1.35027456541736, 1.11503741384757 ), Pool_21.sf = c(1.06155444204363, 0.991570644521018), Pool_22.sf = c(1.64504600710891, 1.07862501013554), Pool_23.sf = c(1.51097405304331, 0.971834605384123 ), Pool_24.sf = c(1.18420663655483, 0.881393761143161), Pool_25.sf = c(0.925122055385438, 0.93313232911786), Pool_26.sf = c(2.12016328112954, 0.829308431444176 ), Pool_27.sf = c(1.59552456085871, 0.705278334816745), Pool_28.sf = c(1.75141617967796, 0.863808031900547), Pool_29.sf = c(1.71320920062242, 0.782291400605908 ), Pool_3.sf = c(1.09209110640701, 0.776979928448013), Pool_30.sf = c(0.925564956736256, 0.905870068022084), Pool_31.sf = c(1.00114849632652, 0.713896646497438 ), Pool_32.sf = c(0.769653226223374, 1.01812180736834), Pool_33.sf = c(1.64152662148587, 0.912970524890157), Pool_34.sf = c(1.39446534544181, 0.892464822723893 ), Pool_35.sf = c(1.36553718507047, 0.709121064448927), Pool_36.sf = c(1.48178605247809, 0.766690878721894), Pool_37.sf = c(1.05050355917415, 0.862090327153509 ), Pool_38.sf = c(1.36989138311191, 1.01473830511752), Pool_39.sf = c(1.42872045770954, 1.13176474162602), Pool_4.sf = c(1.11960747784989, 0.665514805707436 ), Pool_40.sf = c(1.90897625098439, 1.28419857359682), Pool_41.sf = c(1.2570145072185, 0.987813170293439), Pool_43.sf = c(1.14927112622372, 1.33241620047574 ), Pool_44.sf = c(1.02884805988699, 1.1077339415536), Pool_45.sf = c(1, 1), Pool_46.sf = c(1.15692580371101, 1.01663753799148), Pool_47.sf = c(1.02161799920975, 0.893420612254083), Pool_48.sf = c(0.991350522776138, 0.857531005677309 ), Pool_49.sf = c(0.666054364361721, 0.95128169066564), Pool_5.sf = c(1.27677591889858, 0.65869398169343), Pool_50.sf = c(1.04592846997826, 0.820965050229932 ), Pool_51.sf = c(1.46227623256989, 1.16138433421938), Pool_52.sf = c(1.1826746106421, 1.33429257056276), Pool_53.sf = c(1.16041540250292, 0.878127525893012 ), Pool_54.sf = c(1.14285567434696, 0.870429885808645), Pool_55.sf = c(1.40863161629042, 0.485422488325543), Pool_56.sf = c(1.81157566249543, 0.519084970767436 ), Pool_58.sf = c(1.69798017279487, 1.34651488521988), Pool_59.sf = c(1.55336058362464, 0.982570924872293), Pool_6.sf = c(0.769881996423631, 0.388931536871056 ), Pool_60.sf = c(1.36877679300045, 1.02579768967408), Pool_61.sf = c(1.34960258409398, 0.983191813100761), Pool_62.sf = c(1.08159654058587, 0.76318904250517 ), Pool_63.sf = c(1.98209270942409, 1.05152970776951), Pool_64.sf = c(1.86946484050877, 1.06489241167699), Pool_65.sf = c(1.48159508541161, 0.89626404845365 ), Pool_66.sf = c(1.42400489307256, 1.30732410445944), Pool_7.sf = c(1.17869553929846, 0.620813490764102), Pool_8.sf = c(1.35783021860687, 0.77620120083204 ), Pool_9.sf = c(1.32884787662603, 0.758057408306258)), row.names = c("Glyma.01G000400", "Glyma.01G000900"), class = "data.frame")
df1 %>%
rownames_to_column() %>%
pivot_longer(cols = -c(rowname)) %>%
mutate(name = factor(name, levels = unique(name))) %>%
ggplot(aes(x = name, y = value, group = rowname, color = rowname)) +
geom_line() +
theme(axis.text.x = element_text(angle = 45, hjust = 1.05))
I get this plot:

Is this what you want? Otherwise, what do you want to change?