Borrowing all the code from Multithreading With Python Turtle, this appears to "work" for getting both functions to run at the same time.
If so, this is likely better a duplicate of linked answer.
import queue
import threading
import turtle
a = turtle.Screen()
b = turtle.Turtle()
c = turtle.Turtle()
def one():
b.forward(100)
def two():
c.forward(-100)
def process_queue():
while not graphics.empty():
(graphics.get())(1)
if threading.active_count() > 1:
turtle.ontimer(process_queue, 100)
graphics = queue.Queue(1) # size = number of hardware threads you have - 1
turtle1 = turtle.Turtle('turtle')
turtle1.speed('fastest')
thread1 = threading.Thread(target=one)
thread1.daemon = True # thread dies when main thread (only non-daemon thread) exits.
thread1.start()
turtle2 = turtle.Turtle('turtle')
turtle2.speed('fastest')
thread2 = threading.Thread(target=two)
thread2.daemon = True # thread dies when main thread (only non-daemon thread) exits.
thread2.start()
turtle.mainloop()