With float arithmetics you should always have the fact of Is floating math broken - which is documented here: python.org "15. Floating Point Arithmetic: Issues and Limitations" in the back of your mind.
Especially if you handle very tiny and very big numbers together.
You have numbers that are 1e36 apart ... your result differs by a factor of 50.
Float math does not handle very big/very small values very good - especially not if you mix them - it introduces rounding errors.
Edit:
Conversion to fractions of the initial values does lead to a difference, but it is tiny - so probably not broken floating math at work here:
from fractions import Fraction
from math import pi
C_gamma = 8.846E-14
Gamma = 11741.707101355101
beamEnergy = 6.0E9
I2 = 0.2803660599555248
E0 = (beamEnergy/Gamma)
U0 = E0**4 * I2 * C_gamma/(2*pi)
print(f"{U0:20.15f}")
fr_C_gamma = Fraction.from_float( 8.846E-14)
fr_Gamma = Fraction.from_float(11741.707101355101)
fr_beamEnergy = Fraction.from_float(6.0E9 )
fr_I2 = Fraction.from_float(0.2803660599555248)
fr_E0 = fr_beamEnergy/fr_Gamma
fr_U0 = fr_E0**4 * fr_I2 * fr_C_gamma/(2*pi)
print(f"{fr_U0:20.15f}")
Output:
269136460.219558060169220 (float)
269136460.219558119773865 (Fractions)
So mabye you should go over the initial formula again and see if that has some problems - as pointed out by Robert Dodier in a comment your formula contains m/GeV3
- which may be Volt cubed?