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I have tried to set up Kallisto software - following the instructions on https://protocols.hostmicrobe.org/conda without any luck. I need this software to align reads to a reference genome for RNA sequencing analysis. It worked up until I used the command: conda create --name rnaseq on anaconda prompt (miniconda3) as it came up as an error.

Does anyone know how I might be able to correct this?

Thanks

bioinformatics_
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  • Can you please include the error from anaconda as text. This would really help in solving your problem. – Mime Jul 06 '22 at 07:23
  • Thankyou for your response. Apologises I will be able to post it in 2 hours as I'm currently on a different computer. FYI I'm working on a windows computer. – bioinformatics_ Jul 06 '22 at 07:27
  • Collecting package metadata (current_repodata.json): done Solving environment: failed with initial frozen solve. Retrying with flexible solve. Collecting package metadata (repodata.json): done Solving environment: failed with initial frozen solve. Retrying with flexible solve. PackagesNotFoundError: The following packages are not available from current channels: - kallisto – bioinformatics_ Jul 06 '22 at 10:48
  • PackagesNotFoundError: The following packages are not available from current channels: - kallisto Current channels: - https://conda.anaconda.org/bioconda/win-64 - https://conda.anaconda.org/bioconda/noarch - https://conda.anaconda.org/conda-forge/win-64 - https://conda.anaconda.org/conda-forge/noarch - https://repo.anaconda.com/pkgs/main/win-64 – bioinformatics_ Jul 06 '22 at 10:49
  • - https://repo.anaconda.com/pkgs/main/noarch - https://repo.anaconda.com/pkgs/r/win-64 - https://repo.anaconda.com/pkgs/r/noarch - https://repo.anaconda.com/pkgs/msys2/win-64 - https://repo.anaconda.com/pkgs/msys2/noarch To search for alternate channels that may provide the conda package you're looking for, navigate to https://anaconda.org and use the search bar at the top of the page. – bioinformatics_ Jul 06 '22 at 10:49
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    Welcome to StackOverflow. Please incorporate additional information by editing the question rather than posting in comments. Comments are great way to alert users of when changes are made to the question, but not good for information that would clarify the original question by being included. – merv Jul 06 '22 at 16:24
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    Bioconda does not support Windows. – merv Jul 06 '22 at 16:27

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