I have an R coding question.
This is my first time asking a question here, so apologies if I am unclear or do something wrong. I am trying to use a Generalized Linear Mixed Model (GLMM) with Poisson error family to test for any significant effect on a count response variable by three separate dichotomous variables (AGE = ADULT or JUVENILE, SEX = MALE or FEMALE and MEDICATION = NEW or OLD) and an interaction between AGE and MEDICATION (AGE:MEDICATION). There is some dependency in my data in that the data was collected from a total of 22 different sites (coded as SITE vector with 33 distinct levels), and the data was collected over a total of 21 different years (coded as YEAR vector with 21 distinct levels, and treated as a categorical variable). Unfortunately, every SITE was not sampled for each YEAR, with some being sampled for a greater number of years than others. The data is also quite sparse, in that I do not have a great number of measurements of the response variable (coded as COUNT and an integer vector) per SITE per YEAR.
My Poisson GLMM is constructed using the following code:
model <- glmer(data = mydata,
family = poisson(link = "log"),
formula = COUNT ~ SEX + SEX:MEDICATION + AGE + AGE:SEX + MEDICATION + AGE:MEDICATION + (1|SITE/YEAR),
offset = log(COUNT.SAMPLE.SIZE),
nAGQ = 0)
In order to try and obtain more reliable estimates for the fixed effect coefficients (particularly given the sparse nature of my data), I am trying to obtain 95% confidence intervals for the fixed effect coefficients through non-parametric bootstrapping.
I have come across the "glmmboot" package which can be used to conduct non-parametric bootstrapping of GLMMs, however when I try to run the non-parametric bootstrapping using the following code:
library(glmmboot)
bootstrap_model(base_model = model,
base_data = mydata,
resamples = 1000)
When I run this code, I receive the following message:
Performing case resampling (no random effects)
Naturally, though, my model does have random effects, namely (1|SITE/YEAR). If I try to tell the function to resample from a specific block, by adding in the "reample_specific_blocks" argument, i.e.:
library(glmmboot)
bootstrap_model(base_model = model,
base_data = mydata,
resamples = 1000,
resample_specific_blocks = "YEAR")
Then I get the following error message:
Performing block resampling, over SITE Error: Invalid grouping factor specification, YEAR:SITE
I get a similar error message if I try set 'resample_specific_blocks' to "SITE". If I then try to set 'resample_specific_blocks' to "SITE:YEAR" or "SITE/YEAR" I get the following error message:
Error in bootstrap_model(base_model = model, base_data = mydata, resamples = 1000, : No random columns from formula found in resample_specific_blocks
I have tried explicitly nesting YEAR within SITE and then adapting the model accordingly using the code:
mydata <- within(mydata, SAMPLE <- factor(SITE:YEAR))
model.refit <- glmer(data = mydata,
family = poisson(link = "log"),
formula = COUNT ~ SEX + AGE + MEDICATION + AGE:MEDICATION + (1|SITE) + (1|SAMPLE),
offset = log(COUNT.SAMPLE.SIZE),
nAGQ = 0)
bootstrap_model(base_model = model.refit,
base_data = mydata,
resamples = 1000,
resample_specific_blocks = "SAMPLE")
But unfortunately I just get this error message:
Error: Invalid grouping factor specification, SITE
The same error message comes up if I set resample_specific_blocks argument to SITE, or if I just remove the resample_specific_blocks argument.
I believe that the case_bootstrap() function found in the lmeresampler package could potentially be another option, but when I look into the help for it it looks like I would need to create a function and I unfortunately have no experience with creating my own functions within R.
If anyone has any suggestions on how I can get the bootstrap_model() function in the glmmboot package to recognise the random effects in my model/dataframe, or any suggestions for alternative methods on conducting non-parametric bootstrapping to create 95% confidence intervals for the coefficients of the fixed effects in my model, it would be greatly appreciated! Many thanks in advance, and for reading such a lengthy question!
For reference, I include links to the RDocumentation and GitHub for the glmmboot package: https://www.rdocumentation.org/packages/glmmboot/versions/0.6.0
https://github.com/ColmanHumphrey/glmmboot
The following is code that will allow for creation of a reproducible example using the data set from lme4::grouseticks
#Load in required packages
library(tidyverse)
library(lme4)
library(glmmboot)
library(psych)
#Load in the grouseticks dataframe
data("grouseticks")
tibble(grouseticks)
#Create dummy vectors for SEX, AGE and MEDICATION
set.seed(1)
SEX <-sample(1:2, size = 403, replace = TRUE)
SEX <- as.factor(ifelse(SEX == 1, "MALE", "FEMALE"))
set.seed(2)
AGE <- sample(1:2, size = 403, replace = TRUE)
AGE <- as.factor(ifelse(AGE == 1, "ADULT", "JUVENILE"))
set.seed(3)
MEDICATION <- sample(1:2, size = 403, replace = TRUE)
MEDICATION <- as.factor(ifelse(MEDICATION == 1, "OLD", "NEW"))
grouseticks$SEX <- SEX
grouseticks$AGE <- AGE
grouseticks$MEDICATION <- MEDICATION
#Use the INDEX vector to create a vector of sample sizes per LOCATION
#per YEAR
grouseticks$INDEX <- 1
sample.sizes <- grouseticks %>%
group_by(LOCATION, YEAR) %>%
summarise(SAMPLE.SIZE = sum(INDEX))
#Combine the dataframes together into the dataframe to be used in the
#model
mydata$SAMPLE.SIZE <- as.integer(mydata$SAMPLE.SIZE)
#Create the Poisson GLMM model
model <- glmer(data = mydata,
family = poisson(link = "log"),
formula = TICKS ~ SEX + SEX + AGE + MEDICATION + AGE:MEDICATION + (1|LOCATION/YEAR),
nAGQ = 0)
#Attempt non-parametric bootstrapping on the model to get 95%
#confidence intervals for the coefficients of the fixed effects
set.seed(1)
Model.bootstrap <- bootstrap_model(base_model = model,
base_data = mydata,
resamples = 1000)
Model.bootstrap
