I have a question regarding the picard markduplicates tool. I almost always get very high numbers in the estimated_library_size field (160-190 million) that is nothing like expected library size or the number of reads I get from the sequencing facility. I think I am missing something related to how it is calculated (even though I read the equation used). Could someone please let me know around what values this should be and why do I get these huge numbers as library size if it's correct?
Thanks in advance
