All those clowns glibly posting that "HTTP Error 403.14 - Forbidden: The Web server is configured to not list the contents of this directory" can be fixed by configuring the web site by either enabling the Directory Browsing feature or adding a default document are really not understanding what they are talking about. Anyone can copy a solution from one post and paste to another.
I'm not going to pretend I know what I am talking about but I have fixed my issue and will share what I did here to see in the hope it helps others.
I created a project (of the same type as the application I was trying to deploy) using my IDE (MSVS) in the same .Net version of the application I was trying to deploy. In this case it was creation of an MVC project in .Net Framework 4.5.
I did nothing to it. All I wanted was a skeleton.
I replaced all the files in the home directory of the web site I had an issue with, with the files of this skeleton project.
I attempted to browse to the web site and it worked successfully. Note the directory browsing was not enabled nor was a default directory set.
This proved to me that those settings were irrelevant.
Having googled around, some sites point to paths in config files causing an issue if they do not exist, eg a logging path to d:\logs where that directory was missing. It didn't fix my issue but there's no harm checking.
I stumbled across a post which talked about the roles and features enabled on the server. In Web Server (IIS)/Web Server/Application Development they had selected "Application Initialisation" and "ASP".
The Windows Sever 2012 server I was moving the application from, where it work without a hitch, did not have those selected so had not selected them on the Windows Server 2019 I was moving them to when I set that server up.
It made no sense that this would be the solution but having tried everything else I activated those two options.
Stone me if the issue was resolved.
As a check I removed those two options and the problem came back and when I reselected them it went away again.
This struck me as pretty conclusive only having thought I'd found the cause of the problem I built the website from scratch and the problem came back again.
It took a number of iterations but eventually I got there, more by luck than judgement and whatever the cause was it was due to an issue in the application which wasn't being logged in event viewer so focus on permissions to files being written to: not only that the app pool account is set up on the directories it needs access to but also that it has modify or even full control.
All those muppets robotically posting advice on configuring the web site by either enabling the Directory Browsing feature or adding a default document will no doubt copy this and paste it into their posts to claim the credit but c'est la vie.
I hope this post is of use to someone, apart from those muppets.