Yes.
Both of these are practices to be avoided where possible.
Applying almost any function to a column makes the expression unsargable which means an index cannot be used and even if the column is not indexed it makes cardinality estimates incorrect for the rest of the plan.
Additionally your dbo.GetLookupDetailTitle
scalar function looks like it does data access and this should be inlined into the query.
The query optimiser does not inline logic from scalar UDFs and your query will be performing this lookup for each row in your source data, which will effectively enforce a nested loops join irrespective of its suitability.
Additionally this will actually happen twice per row because of the 2 function invocations. You should probably rewrite as something like
SELECT M.* /*But don't use * either, list columns explicitly... */
FROM Member M
WHERE NOT EXISTS(SELECT *
FROM MemberRoles R
WHERE R.MemberId = M.MemberId
AND R.RoleId IN (1,2)
)
Don't be tempted to replace the literal values 1,2
with variables with more descriptive names as this too can mess up cardinality estimates.