AttributeError: module 'collections' has no attribute 'Sequence'
i get this error everything i try to run my code but there isn't any information about how to use Mothur except for the the documentation.
`# python3
import sys import queue import itertools from collections import deque from mothur_py import Mothur import collections.abc as collections
class KmerIdMgmt:
def __init__(self):
self.id = 0
self.ids_map = {}
self.kmers = {}
def insert(self, kmer):
if kmer not in self.ids_map:
self.ids_map[kmer] = self.id
self.kmers[self.id] = kmer
self.id += 1
return self.ids_map[kmer]
class DeBruijnGraph(object):
def __init__(self, k, reads):
self.k = k
self.threshold = self.k + 1
self.kmer_ids = KmerIdMgmt()
self.coverage = {}
self.graph = {}
self.outgoing_num = lambda k: len(self.graph[k][0])
self.incoming_num = lambda k: self.graph[k][1]
self.make_deBruijn_graph(self.break_reads_into_kmers(reads))
def break_reads_into_kmers(self, reads):
break_read = lambda read: [ read[j:j + self.k] for j in range(len(read) - self.k + 1) ]
return [ kmer for read in reads for kmer in break_read(read) ]
def make_deBruijn_graph(self, kmers):
def add_edge(graph, coverage, left, right):
graph.setdefault(left, [set(), 0])
graph.setdefault(right, [set(), 0])
coverage.setdefault((left, right), 0)
coverage[(left, right)] += 1
if right not in graph[left][0]:
graph[left][0].add(right)
graph[right][1] += 1
for kmer in kmers:
left = self.kmer_ids.insert(kmer[:-1])
right = self.kmer_ids.insert(kmer[1:])
if left != right:
add_edge(self.graph, self.coverage, left, right)
def remove_leaves(self):
removable = [ k for k, v in self.graph.items() if len(v[0]) == 0 ]
for k in removable:
del self.graph[k]
def print_graph(self):
for k, v in self.graph.items():
print(k, v)
class TipRemoval(DeBruijnGraph):
def __init__(self, k, reads):
DeBruijnGraph.__init__(self, k, reads)
def remove_tips(self):
for k, v in self.graph.items():
find_and_remove = None
if self.outgoing_num(k) == 1 and self.incoming_num(k) == 0:
find_and_remove = self.find_and_remove_incoming
elif self.outgoing_num(k) > 1:
find_and_remove = self.find_and_remove_outgoing
else: continue
condition = True
while condition:
condition = False
for edge in v[0]:
if find_and_remove(edge, 0):
v[0].remove(edge)
condition = True
break
def find_and_remove_outgoing(self, current, depth):
if self.outgoing_num(current) > 1 or self.incoming_num(current) > 1:
return False
if depth == self.threshold:
return False
if self.outgoing_num(current) == 0:
return True
if self.find_and_remove_outgoing(next(iter(self.graph[current][0])), depth + 1):
to = next(iter(self.graph[current][0]))
self.graph[current][0].pop()
self.graph[to][1] -= 1
return True
return False
def find_and_remove_incoming(self, current, depth):
if self.outgoing_num(current) == 0 or self.incoming_num(current) > 1:
return True
if depth == self.threshold:
return False
if self.find_and_remove_incoming(next(iter(self.graph[current][0])), depth + 1):
to = next(iter(self.graph[current][0]))
self.graph[current][0].pop()
self.graph[to][1] -= 1
return True
return False
class BubbleRemoval(TipRemoval):
def __init__(self, k, reads):
TipRemoval.__init__(self, k, reads)
self.paths = {}
def remove_bubbles(self):
for k, v in self.graph.items():
if self.outgoing_num(k) > 1:
self.dfs(path=[k], current=k, depth=0)
for pair, candidates_list in self.paths.items():
source, target = pair[0], pair[1]
best_path = max(candidates_list, key=lambda item: item[1])[0]
for path, _ in candidates_list:
if best_path == path or not self.bubble_possible(source, target):
continue
if self.paths_disjoint(best_path, path) and self.path_exists(path):
self.remove_path(path)
def bubble_possible(self, source, target):
return len(self.graph[source][0]) > 1 and self.graph[target][1] > 1
def path_exists(self, path):
for j in range(len(path) -1):
if path[j +1] not in self.graph[path[j]][0]:
return False
return True
def remove_path(self, path):
for j in range(len(path) -1):
self.graph[path[j]][0].remove(path[j +1])
self.graph[path[j +1]][1] -= 1
del self.coverage[(path[j], path[j +1])]
def paths_disjoint(self, a, b):
return len(set(a) & set(b)) == 2
def dfs(self, path, current, depth):
if current != path[0] and self.incoming_num(current) > 1:
weight = sum(self.coverage[(path[i], path[i+1])] for i in range(len(path)-1)) / len(path)
self.paths.setdefault((path[0], current), list()).append((path[:], weight))
if depth == self.threshold:
return
for next_ in self.graph[current][0]:
if next_ not in path:
path.append(next_)
self.dfs(path, next_, depth + 1)
path.remove(next_)
class PhiX174GenomeAssembler(BubbleRemoval):
def __init__(self, k, reads):
BubbleRemoval.__init__(self, k, reads)
def make_Euler_cycle(self):
verteces = deque()
path = []
# line 191
current = next(iter(self.graph))
verteces.append(current)
while verteces:
current = verteces[0]
if len(self.graph[current][0]) != 0:
t = next(iter(self.graph[current][0]))
verteces.append(t)
self.graph[current][0].remove(t)
continue
path.append(current)
verteces.popleft()
return path
def assemble(self):
self.remove_tips()
self.remove_leaves()
self.remove_bubbles()
cycle = self.make_Euler_cycle()
circular_genome = self.kmer_ids.kmers[cycle[0]]
for i in range(1, len(cycle) - (self.k - 1)):
circular_genome += self.kmer_ids.kmers[cycle[i]][-1]
return circular_genome
if __name__ == "__main__":
n_kmers = int(input())
for _ in range(n_kmers):
reads = list(input())
reads = str(reads)
with open('reads.fasta', 'w') as read:
read.write(reads)
k = 100
m = Mothur()
contig = m.make.contigs(ffasta = read)
for x in range(n_kmers):
print(">CONTIG", x)
print(contig)
`
