One way would be to prepare a CSV file with all the values that you will need. There are a multitude of different ways to use it afterwards. Alies Belik's answer listed most of them. The drawback of the CSV approach, however, is that you need to generate the list of values, and in some tests you can't simply reuse it without cleaning up/reinitializing the back-end database.
Another option are the functions for generating random values, usually paired with "User Defined Variables" controller.
- __Random for generating numbers in a given range.
- __RandomString for generating random strings of a given length and containing a set of characters.
This is a powerful mechanism, but I find it somewhat cumbersome and clunky.
For simple variables, like generating username/password/e-mail combinations, I prefer and find it easier to use the Random Variable config element. It's available since Jmeter 2.3.3. You add it to your thread group and specify a variable to store the random value for each thread. You can later reference this variable in your HTTP sampler, in the GET/POST parameters of the request, by specifying the Value of the parameter to be testuser-${rnd}
for username, testpass-${rnd}
for password. Each thread will get a different value of ${rnd}
so there is a small chance (but there is still a chance) that you will get duplicate values (users).