Questions tagged [imagej-hyperstack]

I would like to convert several TIFF files with 32-bit pixel depth into 8-bit pixel depth TIFFs while retaining metadata and TIFF tags. The 32-bit TIFFs are four-dimensional ImageJ-hyperstacks with TZYX axes (i.e. time, z-depth, y-coordinate,…

Is there a way of how to subtract one stack of images from another stack using FIJI (ImageJ)? To be more specific, I would like to find a quick way for such a subtraction, where first image of the first stack is subtracted from the first image of…

ImageJ HyperStacks have a data type (8-32 bit), a width, a height, a number of channels, a number of slices and a number of frames. They represent 5D (XYCZT) data sets. ImageJ stores them as multipage tiff files where number of channels times number…

I have ten tiff files and each of them contains two channels imaging data, I want to labeling different colors for the two channels and after that do a z projection, anyone knows how to do it?