SLC9A3R2
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesSLC9A3R2, E3KARP, NHE3RF2, NHERF-2, NHERF2, OCTS2, SIP-1, SIP1, TKA-1, Sodium-hydrogen exchange regulatory cofactor 2, SLC9A3 regulator 2
External IDsOMIM: 606553 MGI: 1890662 HomoloGene: 56962 GeneCards: SLC9A3R2
Orthologs
SpeciesHumanMouse
Entrez

9351

65962

Ensembl

ENSG00000065054

ENSMUSG00000002504

UniProt

Q15599

Q9JHL1

RefSeq (mRNA)

NM_001130012
NM_001252073
NM_001252075
NM_001252076
NM_004785

NM_023055
NM_023449
NM_001357796

RefSeq (protein)

NP_001123484
NP_001239002
NP_001239004
NP_001239005
NP_004776

NP_075542
NP_075938
NP_001344725

Location (UCSC)Chr 16: 2.03 – 2.04 MbChr 17: 24.86 – 24.87 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Sodium-hydrogen exchange regulatory cofactor NHE-RF2 (NHERF-2) also known as tyrosine kinase activator protein 1 (TKA-1) or SRY-interacting protein 1 (SIP-1) is a protein that in humans is encoded by the SLC9A3R2 (solute carrier family 9 isoform A3 regulatory factor 2) gene.[5][6]

NHERF-2 is a scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. It is necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. In addition, it may also act as scaffold protein in the nucleus.

Function

This regulatory protein (factor) interacts with a sodium/hydrogen exchanger NHE3 (SLC9A3) in the brush border membrane of the proximal tubule, small intestine, and colon that plays a major role in transepithelial sodium absorption. SLC9A3R2, as well as SLC9A3R1 and protein kinase A phosphorylation, may play a role in NHE3 regulation.[7]

Interactions

Sodium-hydrogen exchange regulatory cofactor 2 has been shown to interact with SGK,[8][9] Actinin alpha 4,[10] Parathyroid hormone receptor 1,[11] Phosphoinositide-dependent kinase-1,[8] EZR,[12][13] PODXL,[14][15][16] Cystic fibrosis transmembrane conductance regulator[17] and PLCB3.[18]

See also

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000065054 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000002504 - Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. Poulat F, de Santa Barbara P, Desclozeaux M, Soullier S, Moniot B, Bonneaud N, Boizet B, Berta P (March 1997). "The human testis determining factor SRY binds a nuclear factor containing PDZ protein interaction domains". J. Biol. Chem. 272 (11): 7167–72. doi:10.1074/jbc.272.11.7167. PMID 9054412.
  6. Hall RA, Ostedgaard LS, Premont RT, Blitzer JT, Rahman N, Welsh MJ, Lefkowitz RJ (July 1998). "A C-terminal motif found in the beta2-adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na+/H+ exchanger regulatory factor family of PDZ proteins". Proc. Natl. Acad. Sci. U.S.A. 95 (15): 8496–501. Bibcode:1998PNAS...95.8496H. doi:10.1073/pnas.95.15.8496. PMC 21104. PMID 9671706.
  7. "Entrez Gene: SLC9A3R2 solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2".
  8. 1 2 Chun J, Kwon Taegun, Lee Eunjung, Suh Pann-Ghill, Choi Eui-Ju, Sun Kang Sang (October 2002). "The Na(+)/H(+) exchanger regulatory factor 2 mediates phosphorylation of serum- and glucocorticoid-induced protein kinase 1 by 3-phosphoinositide-dependent protein kinase 1". Biochem. Biophys. Res. Commun. United States. 298 (2): 207–15. doi:10.1016/S0006-291X(02)02428-2. ISSN 0006-291X. PMID 12387817.
  9. Yun CC, Chen Yueping, Lang Florian (March 2002). "Glucocorticoid activation of Na(+)/H(+) exchanger isoform 3 revisited. The roles of SGK1 and NHERF2". J. Biol. Chem. United States. 277 (10): 7676–83. doi:10.1074/jbc.M107768200. ISSN 0021-9258. PMID 11751930.
  10. Kim JH, Lee-Kwon W, Park JB, Ryu SH, Yun CH, Donowitz M (June 2002). "Ca(2+)-dependent inhibition of Na+/H+ exchanger 3 (NHE3) requires an NHE3-E3KARP-alpha-actinin-4 complex for oligomerization and endocytosis". J. Biol. Chem. United States. 277 (26): 23714–24. doi:10.1074/jbc.M200835200. ISSN 0021-9258. PMID 11948184.
  11. Mahon MJ, Donowitz Mark, Yun C Chris, Segre Gino V (June 2002). "Na(+)/H(+ ) exchanger regulatory factor 2 directs parathyroid hormone 1 receptor signalling". Nature. England. 417 (6891): 858–61. Bibcode:2002Natur.417..858M. doi:10.1038/nature00816. ISSN 0028-0836. PMID 12075354. S2CID 4379134.
  12. Yun CH, Lamprecht G, Forster D V, Sidor A (October 1998). "NHE3 kinase A regulatory protein E3KARP binds the epithelial brush border Na+/H+ exchanger NHE3 and the cytoskeletal protein ezrin". J. Biol. Chem. UNITED STATES. 273 (40): 25856–63. doi:10.1074/jbc.273.40.25856. ISSN 0021-9258. PMID 9748260.
  13. Sitaraman SV, Wang Lixin, Wong Michelle, Bruewer Matthias, Hobert Michael, Yun C-H, Merlin Didier, Madara James L (September 2002). "The adenosine 2b receptor is recruited to the plasma membrane and associates with E3KARP and Ezrin upon agonist stimulation". J. Biol. Chem. United States. 277 (36): 33188–95. doi:10.1074/jbc.M202522200. ISSN 0021-9258. PMID 12080047.
  14. Meder D, Shevchenko Anna, Simons Kai, Füllekrug Joachim (January 2005). "Gp135/podocalyxin and NHERF-2 participate in the formation of a preapical domain during polarization of MDCK cells". J. Cell Biol. United States. 168 (2): 303–13. doi:10.1083/jcb.200407072. ISSN 0021-9525. PMC 2171597. PMID 15642748.
  15. Takeda T, McQuistan T, Orlando R A, Farquhar M G (July 2001). "Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton". J. Clin. Invest. United States. 108 (2): 289–301. doi:10.1172/JCI12539. ISSN 0021-9738. PMC 203027. PMID 11457882.
  16. Li Y, Li Jian, Straight Samuel W, Kershaw David B (June 2002). "PDZ domain-mediated interaction of rabbit podocalyxin and Na(+)/H(+) exchange regulatory factor-2". Am. J. Physiol. Renal Physiol. United States. 282 (6): F1129–39. doi:10.1152/ajprenal.00131.2001. ISSN 0363-6127. PMID 11997330.
  17. Sun F, Hug M J, Lewarchik C M, Yun C H, Bradbury N A, Frizzell R A (September 2000). "E3KARP mediates the association of ezrin and protein kinase A with the cystic fibrosis transmembrane conductance regulator in airway cells". J. Biol. Chem. UNITED STATES. 275 (38): 29539–46. doi:10.1074/jbc.M004961200. ISSN 0021-9258. PMID 10893422.
  18. Hwang JI, Heo K, Shin K J, Kim E, Yun C, Ryu S H, Shin H S, Suh P G (June 2000). "Regulation of phospholipase C-beta 3 activity by Na+/H+ exchanger regulatory factor 2". J. Biol. Chem. UNITED STATES. 275 (22): 16632–7. doi:10.1074/jbc.M001410200. ISSN 0021-9258. PMID 10748023.

Further reading

  • Overview of all the structural information available in the PDB for UniProt: Q15599 (Na(+)/H(+) exchange regulatory cofactor NHE-RF2) at the PDBe-KB.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.